Representation of detected glycoforms on the N-184 glycosylation site of 21K slow prion strain.

A) Extracted ion chromatograms with mutual 46 glycoforms detected. B) Assigned glycoforms in MS spectrum with N-184 peptide backbone: neutral, C) monosialylated and D) disialylated (together with one trisialylated glycoform). Blue square–N-acetylglucosamine (GlcNAc), green circle–mannose (Man), red triangle–fucose (Fuc), yellow circle–galactose (Gal), purple diamond–N-acetylneuraminic acid (Neu5Ac).