Reconstitution of IFN-γ responsiveness upon the expression of cDNA1

Copyright information:

Taken from "Screening of retroviral cDNA libraries for factors involved in protein phosphorylation in signaling cascades"

Nucleic Acids Research 2005;33(4):e39-e39.

Published online 24 Feb 2005

PMCID:PMC549578.

© The Author 2005. Published by Oxford University Press. All rights reserved

2. () Detection of pStat1 in unstimulated and IFN-γ-stimulated cells transduced with BMN-I-GFP and BMN-I-GFP-cDNA1.2, respectively. Numbers represent the percentage of cells within each quadrant. () Western-blot analysis of cell lysates of wt U937, naive U937 clone #10.13, BMN-I-GFP transduced #10.13 and BMN-I-GFP-cDNA1.2 transduced unstimulated cells (−) and after IFN-γ stimulation (+). Antibodies raised against Stat1 (upper panel) and pStat1 (pY701; lower panel).