Reanalysis of KIR-adjusted HLA-C*07:02/IE-1-specific T cell frequencies.

HLA-C*07:02/IE-1 multimer⁺ CD8⁺ T cell frequencies for the donors from Fig 1 were adjusted for epitope-independent KIR-binding by excluding cells with HLA-C*07:02/MAGE multimer co-staining. (A) Multimer stainings of HLA-C*07:02/IE-1^MAGE-- and HLA-B*07:02/pp65-specific CD8⁺ T cells are compared. (B) Regression analysis of HLA-C*07:02/IE-1^MAGE- multimer⁺ and IFNγ⁺ HLA-C*07:02/IE-1 peptide-stimulated CD8⁺ T cells. T cells were uniformly pre-gated on living CD3⁺/CD8⁺ lymphocytes. Statistical analysis was performed with the Mann-Whitney U test. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.