Reactive oxygen species (ROS) measurement in human brain microvascular endothelial cells (hCMEC/D3) after 4-hour treatment with 0.01–10 μM compound 8 in the absence and presence of oxidative stress promoted by tert-butyl hydroperoxide (tBHP, 350 μM).

Data is given in fluorescent intensity corresponding to ROS amount produced intracellularly measured by DCFDA assay. Data are presented as mean ± standard deviation (SD; n = 7–8). Data analysis was performed using one-way analysis of variance (ANOVA) followed by Bonferroni’s multiple comparisons test. Statistical significance: ***p < 0.001, compared to the control group, ^##p < 0.01; ^###p < 0.001, compared to the tBHP group.