Rbcn-3A is essential for Yl recycling and yolk granule biogenesis.

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with oocyte-specific expression of dsRNA against Rbcn-3A, double-labeled with phalloidin (red) and antibody against (A, B) endogenous Rab7 (green) or (C, D) GFP (green) from flies (C) with ectopic expression of GFP-2xFYVE or (D) carrying a genome-tagging Yl-eGFP-3xHA. Images are presented as overlaying images in color or in individual channels in gray, as annotated. (B-B3, C1, D1) High-magnification views of the cortex regions highlighted in (A, C, D), respectively, as annotated. Genotypes: The samples were from adult females flies heterozygous for both matalpha4-GAL-VP16 driver (BDSC #7062) and the following UAS-transgenic RNAi and PI(3)P or Yl reporter lines: (A,B) P{TRiP.HMS01287}attP2 (#34612). (C) P{w(+mC) = UAS-GFP-myc-2xFYVE}2 (#42172); P{TRiP. HMS01287}attP2 (#34612). (D). P{TRiP.HMS01287}attP2 (#34612); p{mini-W+, yl-eGFP-3xHA}. The sizes of the scale bars as annotated.