pgen.1010012.s001.tif (759.59 kB)
RT-PCR analysis of fbl, ubtf, and ncl expression during X. laevis development.
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posted on 2022-01-18, 18:42 authored by Jonathan Delhermite, Lionel Tafforeau, Sunny Sharma, Virginie Marchand, Ludivine Wacheul, Ruben Lattuca, Simon Desiderio, Yuri Motorin, Eric Bellefroid, Denis L. J. LafontaineTotal RNA extracted from embryos at the indicated stages was analyzed by RT-PCR using amplicons specific to fbl, ubtf, or ncl transcripts (see Materials and Methods).
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six developmental stagesfunctional ribosomal subunitsevery living cellessential nanomachines responsiblediv >< pneural crest cellsncc survival causedindividual embryos indicatedearly developmental defectsrrna 2 ’-fbl knockdown leadsearly embryonic developmentxenopus laevis 18s rrna production2 ’-xenopus laevis rrna processingprotein productionneural platencc derivativesfibrillarin knockdowndeveloping embryos28s rrnax fbl validated orthogonallyusing ribomethseqthree positionsspecific positionsnew onesnccs ),methyltransferase fibrillarinmethylated positionsmassive p53including 10frog developmentefficient predependent apoptosiscraniofacial skeletoncraniofacial developmentcalled ribosomopathiesbox cbioinformatic screening>, confirming
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