ROS in USUV infection.

(A) Vero cells were mock-infected or infected with USUV at an MOI of 1 and then treated with drug vehicle (DMSO) for 4 hours at 48 hpi, or left untreated. CellROX green Reagent was added at a final concentration of 5 μM and incubated for 30 minutes at 37°C, and cells were analyzed by immunofluorescence. Green fluorescent signal showed the ROS-mediated oxidation of the reagent. Nuclei were stained with To-Pro-3 (blue). Scale bars, 25 μm. (B) Quantification of CellROX fluorescent puncta in cells treated as in A.

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