Gkl494f2.jpg (20.35 kB)
RNase footprinting
figure
posted on 2011-12-30, 16:12 authored by Devanathan Raghunathan, Víctor M. Sánchez-Pedregal, Jochen Junker, Claudia Schwiegk, Markus Kalesse, Andreas Kirschning, Teresa CarlomagnoCopyright information:
Taken from "TAR-RNA recognition by a novel cyclic aminoglycoside analogue"
Nucleic Acids Research 2006;34(12):3599-3608.
Published online 19 Jul 2006
PMCID:PMC1524922.
© 2006 The Author(s)
RNase protection assays of the TAR/ complex were carried out with 10 µM 5′-P-labeled HIV-2 TAR-RNA at 4°C. The digestion experiments were analyzed by gel electrophoresis. () Digestion with RNase A (selective for C and U). Lane 1, control without RNase; lane 2, compound (10 mM); lane 3, compound (5 mM); lane 4, compound (500 µM); lane 5, compound (1.2 µM); lane 6, compound (0.12 µM). () Digestion with RNase T1 (G selective). Lane 1, control without RNase; lane 2, compound (10 mM); lane 3, compound (5 mM); lane 4, compound (500 µM); lane 5, compound (1.2 µM); lane 6, compound (0.12 µM).