ppat.1009430.g003.tif (4.26 MB)

RNA-seq analysis of monocytes after Pf-iRBC stimulation reveals a regulatory signature in Malian adults that is distinct from children.

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posted on 2021-04-06, 17:24 authored by Rajan Guha, Anna Mathioudaki, Safiatou Doumbo, Didier Doumtabe, Jeff Skinner, Gunjan Arora, Shafiuddin Siddiqui, Shanping Li, Kassoum Kayentao, Aissata Ongoiba, Judith Zaugg, Boubacar Traore, Peter D. Crompton

Monocytes isolated from PBMCs of Malian children (n = 8) and adults (n = 8) were stimulated in vitro with Pf-iRBC lysate at a monocyte:Pf-iRBC ratio of 1:5 for 24 hours and then total RNA was isolated for sequencing. From separate subjects (n = 4 subjects in each age group), total RNA from unstimulated monocytes was also sequenced. (A) Principal-component analysis of log2-normalized gene counts across all samples. (B-E) Heatmaps representing log2-normalized gene counts of Pf-iRBC stimulated monocytes from Malian children and adults. Each column represents one individual sample. Heatmaps represent gene sets with pre-specified functions: (B) M1/M2 monocyte/macrophage signature, (C) inflammation, (D) phagocytosis, and (E) antigen processing and presentation.