pone.0260878.s005.tif (546.1 kB)
Quantitative analysis of urine of PodoPalld129+/+ and PodoPalld129-/- mice.
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posted on 2021-12-08, 18:29 authored by Nadine Artelt, Alina M. Ritter, Linda Leitermann, Felix Kliewe, Rabea Schlüter, Stefan Simm, Jens van den Brandt, Karlhans Endlich, Nicole EndlichEqual volume of sterile urine from 6- and 12-months old PodoPalld129+/+ and PodoPalld129-/- mice were separated by SDS-Page and were subsequently stained using CBB (Coomassie Brilliant Blue). BSA was used as a charge control (lane 2). No albumin band is seen (dotted outline), in 6 months old PodoPalld129-/- (lane 4–6) as well as in 12 months old PodoPalld129-/- (lane 8–10) indicating no increased proteinuria in the PodoPalld129-/- mice. The major urinary proteins (MUPs) showed a strong signal between 15 kDa and 20 kDa.
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parietal epithelial cells129 genetic backgroundmorphological deviations like12 months showedchronic kidney diseasesbinding protein palladin>, three palladinpodocyte foot processesdiv >< pbinding partners pdlim26 genetic backgroundproper actin cytoskeletonsignificantly reduced expressionproper podocyte morphologypodopalld129 -/- micepodopalld129 -/-podocyte morphologyreduced expressionmorphological changesimportant actinpodopalld129 -/-)kidney dependskidney damageintact morphologypodocyte spacetaken togetherspecific knockoutresults showproteinuria followedpdlim2 marked dilatationinteracting proteinsglomerular tuftfiltration barrierenlarged subclose contactback crossedage showed6 -/-)
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