Qualitative (A) and quantitative RT-PCR (B) analysis of the precursor tRNA^Ile(UAU).

(A) Primer name and design are illustrated in the upper panel, and the results of agarose gel electrophoresis are showed in the lower panel. The PCR products are indicated by arrows. Marker, DNA marker; toto, total cell RNA sample; cyto, cytoplasm; nuc, nucleus; total-RT, total RNA sample without reverse transcriptase in reverse transcription. (B) Quantitative RT-PCR results. Primer name and design are showed in the upper panel, and the corresponding results are showed in the lower panel. Statistical analysis was conducted between the cytoplasm and the nucleus using two-tail Student T test.