ppat.1011879.s002.tiff (2.71 MB)
Purification of the designed immunogens.
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posted on 2024-03-04, 19:36 authored by Rui Ma, Nichole D. Salinas, Sachy Orr-Gonzalez, Brandi Richardson, Tarik Ouahes, Holly Torano, Bethany J. Jenkins, Thayne H. Dickey, Jillian Neal, Junhui Duan, Robert D. Morrison, Apostolos G. Gittis, Justin Y. A. Doritchamou, Irfan Zaidi, Lynn E. Lambert, Patrick E. Duffy, Niraj H. Tolia(A) The size-exclusion chromatography (SEC) profile overlay of the designed immunogens after purification are shown. The yield of each immunogen is illustrated within parenthesis behind the names. The SEC profile of each immunogen was shown below. The results shown here are one representative purification with all purifications performed at least three times with consistent results. (B) SDS-PAGE analysis of the designed immunogens.
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plasmodium falciparum low birth weightfold greater yieldinfected erythrocytes resultsdiv >< pcocktail vaccination strategyguided design leveragingcsa interaction correlateinfected erythrocytescocktail strategyguided designdesign expressedstable immunogenreduced sizepregnancy lossplacental malariamultiple variantsmaternal anemialength structureslength proteincsa ).based immunogens
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