ppat.1010162.g004.tif (2.78 MB)

Pulmonary SARS-CoV-2 infection and macrophage/monocytes in the lung and blood.

figure

posted on 2021-12-20, 18:44 authored by Alexandra Melton, Lara A. Doyle-Meyers, Robert V. Blair, Cecily Midkiff, Hunter J. Melton, Kasi Russell-Lodrigue, Pyone P. Aye, Faith Schiro, Marissa Fahlberg, Dawn Szeltner, Skye Spencer, Brandon J. Beddingfield, Kelly Goff, Nadia Golden, Toni Penney, Breanna Picou, Krystle Hensley, Kristin E. Chandler, Jessica A. Plante, Kenneth S. Plante, Scott C. Weaver, Chad J. Roy, James A. Hoxie, Hongmei Gao, David C. Montefiori, Joseph L. Mankowski, Rudolf P. Bohm, Jay Rappaport, Nicholas J. Maness

A-D. SARS-CoV-2 infection and macrophage infiltration in the lungs of pigtail macaques at 6- (A and B) and 21- days post infection (dpi, C and D). DAPI = White, Green = SARS-CoV-2, Red = IBA1, Blue = Autofluorescence. E and F. Percentage of SARS-CoV-2 infected cells (E) and IBA1+ macrophages (F) in the lung at necropsy. Bars represent median. G and H. Frequency of IL-8 (G) and TNF-⍺ (H) expressing classical monocytes (CD45+ HLA-DR+ CD14- CD16+) in BAL. I-O. Frequencies of Classical (I and L) intermediate (CD45+ HLA-DR+ CD14+ CD16+) (J and M), and non-classical monocytes (CD45+ HLA-DR+ CD14- CD16+) (K and O) in the blood and BAL before and after SARS-CoV-2 infection. Day 0 = day of infection. Panels I-O. Baseline (day -7): n = 3, Day 6: n = 4, Day 14: n = 2, Day 21: n = 2. Kruskal-Wallis comparison of overall means (P_KW) and Dunn’s Multiple comparisons (designated by line, P_D) tests used to determine significance. P values ≤0.05 reported.