pgen.1009774.s001.tif (4.18 MB)

Pten is more bioactive than PTEN in Drosophila, but both suppress PI3K-induced wing overgrowth.

figure

posted on 2021-09-07, 17:45 authored by Payel Ganguly, Landiso Madonsela, Jesse T. Chao, Christopher J. R. Loewen, Timothy P. O’Connor, Esther M. Verheyen, Douglas W. Allan

(A) Representative adult wings from flies of genotype (from top to bottom), omb>attP2, omb>PTEN and omb>dPten. (B) Normalized adult wing size data in a non-PI3K activated background for flies expressing omb>attP2, omb>PTEN, omb>dPten, omb>C124S, omb> G129E and omb> G127R. Adult wing sizes normalized by dividing individual wing area data point by the average of omb>attP2 wing area. (C) Representative adult wings from flies expressing (from top to bottom) omb>PI3K^act+attP2, omb>PI3K^act+PTEN-WT, omb>PI3K^act+dPten. (D) Normalized adult wing size data in a PI3K activated background for flies expressing omb>attP2 (no PI3K), omb>PI3K^act+attP2, omb>PI3K^act+PTEN, omb>PI3K^act+dPten. Adult wing sizes were normalized by dividing individual wing area data points by the average of omb>PI3K^act+attP2 area. Data are expressed as mean ± SD and analyzed using one-way ANOVA with post-hoc Tukey HSD; not significant (ns), * p < 0.05, ** p < 0.01, **** p < 0.0001.