Pseudo-temporal analysis of HNSCC TME.

A) Top ten up-regulated genes of T_regs during the pseudo-time; UMAP presentation illustrating the dynamics of the HNSCC TME and its transition states as they progress through pseudo-time. The lineage tracking initiates at the 0 branch and concludes at the 1 branch. The heatmap depicts genes that experience upregulation within the Treg branch as pseudo-time advances. The highlighted genes comprise the top ten upregulated genes specific to the Treg branch compared to other branches during pseudo-time analysis. B) Venny plot of the top ten up-regulated genes of T_regs and T_regs DEGs; C) The expression pattern of four similar genes between the top ten up-regulated genes of T_regs and T_regs DEGs along the pseudo-time.