Protein purifier instrument.

A) Flow path diagram. Flow paths are colored by buffers (blue), load (orange), pre- and post-pump valves (gray), pre-column waste (purple), though column (red), post-column waste (purple), and fraction collector (pink). B) Front view of the front panel. The four-column configuration shown includes four 5 mL HisTrap^™ Excel columns, four 500 mL loads, and a fraction collector capable of holding 50 mL, 5 mL, and 1 mL centrifuge tubes. Buffer bottles have been partially cropped for clarity. C) Back view of the front panel, showing all the major components.