Protein gels and corresponding western blots of purified recombinant proteins.

(A) Protein gel (left) and corresponding western blot (right) probed for GST showing purified 6 μg GST-CBU1241 and 2 μg GST proteins of approximately expected size, 61.5 kDa and 26 kDa respectively. (B) Protein gel (left) and corresponding western blot (right) probed for 6xHis-tag showing purified 4 μg 6xHis-CBU0823 and 1 μg 6xHis-oMLE, each of the approximately expected sizes of 63.5 kDa and 62.3 kDa respectively, and the lack of detectable 6xHis-tagged product in the mock purification negative control. (C) Western blot probed for 3xFLAG of C. burnetii 0823::Tn pFLAG-CBU0823 whole cell lysate showing C. burnetii expressing a protein product of approximately expected size, 64.5 kDa, from the complementation plasmid. The marker shown is from the corresponding stain-free protein gel.

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