pntd.0011878.s012.tif (293.24 kB)
Propidium iodide staining of one week old human monocyte-derived macrophages (hMDMs) harvested from a healthy donor. The freshly harvested macrophage cells were used to examine the intracellular survival ability of Acanthamoeba-adapted P. aeruginosa (AK1-PA) and wild-type PA-01 strains.
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posted on 2024-01-02, 19:15 authored by Binod Rayamajhee, Mark Willcox, Fiona L. Henriquez, Ajay Kumar Vijay, Constantinos Petsoglou, Gauri Shankar Shrestha, Hari Kumar Peguda, Nicole CarntPropidium iodide staining of one week old human monocyte-derived macrophages (hMDMs) harvested from a healthy donor. The freshly harvested macrophage cells were used to examine the intracellular survival ability of Acanthamoeba-adapted P. aeruginosa (AK1-PA) and wild-type PA-01 strains.
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primary human macrophagesincreasingly important pathogenwild type strainstrain pao1 (<increased neutrophil responsealkyne – functionalizednaturally acquired intracellulardomestic tap waterpseudomonas aeruginosa live intracellular bacteriarats eyes resulteddomestic watermotile strainaeruginosa – alanineclinical responsehousehold waterintracellular survivalintracellular bacteriavivo situ p acanthamoeba various microorganismsspecies recoveredseen throughoutpotentially attributedmetabolically activemay indicategreater degreegenotype t4ffish ),findings indicateenvironmental hostelectron microscopycorneal swabscorneal infectionschallenged intrastromallyanimal modelalso becomingalkdala ).ak patients>< 005 ).
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