pone.0243333.s003.tif (9.2 MB)
Preamplification step optimization.
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posted on 2021-04-14, 17:55 authored by Julien Fassy, Caroline Lacoux, Sylvie Leroy, Latifa Noussair, Sylvain Hubac, Aurélien Degoutte, Georges Vassaux, Vianney Leclercq, David Rouquié, Charles-Hugo Marquette, Martin Rottman, Patrick Touron, Antoinette Lemoine, Jean-Louis Herrmann, Pascal Barbry, Jean-Louis Nahon, Laure-Emmanuelle Zaragosi, Bernard MariThe elongation time used in the preamplification reaction was reduced to 1 min at 60°C (A) from 2 min at 60°C (B) using diluted total RNA from a SARS-nCov2 positive patient sample.
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SARS-CoV -2 variantsoptimized processing bufferassaysetdetectioncapacityinactivating lysis buffersSARS-CoV -2 testing capacitiesRT-qPCRSARS-CoV -2sample PCR reaction volumes4608 qPCR end-pointshigh-throughput SARS-CoV -210 nL-range volumeRNA extraction stepmicrofluidic qPCR testBiomark TM instrumentBiomark TM reactionsresponseprimers
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