Ppg1 controls gluconeogenic flux and outputs in the post-diauxic phase.

A) Relative steady-state amounts of specific gluconeogenic intermediates, precursors of cell wall and storage carbohydrates, and amino acids in WT and ppg1Δ cells after 24hrs of growth in YPD medium. Data represented as a mean ± SD (n = 3). G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; UDP-Glc, uridine diphosphate glucose; UDP-GlcNAc, uridine diphosphate N-acetylglucosamine. *P < 0.05, **P < 0.01, and ***P< 0.001; n.s., non-significant difference, calculated using unpaired Student’s t tests. Also see S2A Fig. B) Relative ¹³C label incorporation in gluconeogenic outputs. WT and ppg1Δ cells were grown in SCD medium for 24hrs, cultures were spiked with ¹³C-acetate for 30mins, and ¹³C label incorporation in gluconeogenic outputs was measured. Data represented as a mean ± SD (n = 6). *P < 0.05, **P < 0.01, and ***P< 0.001; n.s., non-significant difference, calculated using paired Student’s t tests. C) Relative steady-state amounts of specific gluconeogenic outputs from WT and ppg1Δ cells during the course of growth in YPD medium. WT and ppg1Δ cells were grown in YPD medium, and metabolite extraction was carried out at indicated time points. Data represented as a mean ± SD (n = 3). Also see S2B Fig for chitin levels, and S2C and S2D Fig, for growth in the presence of Congo red or Calcofluor white. D) Relative ethanol production by WT and ppg1Δ cells in high-glucose conditions. WT and ppg1Δ cells were grown in YPD medium for 6hrs and ethanol concentration in the media was measured. Data represented as a mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P< 0.001; n.s., non-significant difference, calculated using unpaired Student’s t tests. E) Effect of the loss of Ppg1 on transcript levels of gluconeogenesis, cell wall synthesis and storage carbohydrate synthesis genes. The transcript levels were measured after 24hrs of growth in YPD medium. Data represented as a mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P< 0.001; n.s., non-significant difference, calculated using unpaired Student’s t tests.