pgen.1009113.g002.tif (1.27 MB)

Phylogenic tree of the evolution from primary to recurrent sites.

figure

posted on 2021-01-21, 18:25 authored by Shotaro Sakimura, Satoshi Nagayama, Mitsuko Fukunaga, Qingjiang Hu, Akihiro Kitagawa, Yuta Kobayashi, Takanori Hasegawa, Miwa Noda, Yuta Kouyama, Dai Shimizu, Tomoko Saito, Atsushi Niida, Yusuke Tsuruda, Hajime Otsu, Yoshihiro Matsumoto, Hiroki Uchida, Takaaki Masuda, Keishi Sugimachi, Shin Sasaki, Kazutaka Yamada, Kazuki Takahashi, Hideki Innan, Yutaka Suzuki, Hiromi Nakamura, Yasushi Totoki, Shinichi Mizuno, Masanobu Ohshima, Tatsuhiro Shibata, Koshi Mimori

A) Primary Unique Mut: single nucleotide variants (SNVs) were observed in primary tissues but not in metastatic sites. Primary Trunk Mut: SNVs in both primary tumors and metastatic tumors. M: SNVs in metastatic recurrent cancers only. The list of consensus driver genes is shown. Most driver mutations were observed from primary to metastatic sites; although, CRCR2 (M) was excluded from further analysis because the sample was exposed to multiple chemotherapies for 150 d after the diagnosis of recurrent sites. However, another 10 recurrent tumors were removed within 30 d without any treatment. B) Comparison of the clonality of mutated driver genes between primary and metastatic sites. Most mutated driver genes in primary sites were identical to metastatic sites (neutral evolution). However, a few subclonal mutated driver genes (red arrows) were observed in metastatic sites. C) Comparison of the arm-level CNA between primary and recurrent sites (neutral evolution).