Phylogenetic tree created n based on the patrial NS3 gene sequences of TBEV using the maximum likelihood method and the Tamura-Nei model with a gamma distribution of among-site variation and bootstrap analysis of 1000 replicates (bootstrap values < 50% not shown).

Sample ID or GenBank accession numbers are indicated for each sequence, with the original isolation source, location (if known) and country code. Sequences obtained from TBEV RNA–positive tick samples from seven counties in Lithuania during 2017–2019 are marked: ● –obtained from Ixodes ricinus; ■ –Dermacentor reticulatus. ○ - sequences obtained in Lithuania from ticks by Katargina et al. [18]. The scale indicates the number of nucleotide substitutions per site.