Phenotypic characterization of HLA-C*07:02/IE-1^MAGE- and HLA-B*07:02/pp65 multimer⁺ T cells.

Phenotypic analysis of HLA-C*07:02/IE-1^MAGE-- or HLA-B*07:02/pp65-specific CD8⁺ naïve (CCR7⁺CD45RO^-), central memory (CCR7⁺CD45RO⁺), effector memory (CCR7^-CD45RO⁺) and effector (CCR7^-CD45RO^-) T cells. (A) Exemplary multimer CD8⁺ T cell stainings and (B) differentiation phenotypes for the corresponding epitopes are shown. (C) Central memory, (D) effector memory and (E) effector CD8⁺ T cell subset frequencies (%) from HLA-C*07:02/ HLA-B*07:02 donors are compared for the respective epitopes. T cells were uniformly pre-gated on living CD3⁺/CD8⁺ lymphocytes. Statistical analyses were performed with the Mann-Whitney U test. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.