Phagocytosis of mitochondria by mouse macrophages.

Panels A and B. Fluorescent mitochondria from the liver of mito::mKate2 mice were isolated and incubated with mouse peritoneal macrophages with (Panel A) and without (Panel B) β2-Gp1. The macrophages were stained with Alexa fluor 488-Phalliodin (for cytoplasm). The arrows point to red fluorescent mitochondria within the cells. Panels C and D. Fluorescent red mitochondria from mito::mKate2 mice were incubated with peritoneal macrophages with (Panel C) and without (Panels D) β2-Gp1. The peritoneal macrophages were stained with DAPI (for nucleus) and Lysotracker Green DND-26 (for lysosome). The arrows points to lysosomes (green) and lysosomes colocalized with engulfed mitochondria (yellow). Representative figures of at least 4 or more independent biological replicates.