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Phages Powerpuff, Lego, and YesChef exhibit turbid bullseye plaque morphologies and are Siphoviridae.

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posted on 2022-01-13, 18:30 authored by Andrew Kapinos, Pauline Aghamalian, Erika Capehart, Anya Alag, Heather Angel, Eddie Briseno, Byron Corado Perez, Emily Farag, Hilory Foster, Abbas Hakim, Daisy Hernandez-Casas, Calvin Huang, Derek Lam, Maya Mendez, Ashley Min, Nikki Nguyen, Alexa L. Omholt, Emily Ortiz, Lizbeth Shelly Saldivar, Jack Arthur Shannon, Rachel Smith, Mihika V. Sridhar, An Ta, Malavika C. Theophilus, Ryan Ngo, Canela Torres, Krisanavane Reddi, Amanda C. Freise, Jordan Moberg Parker

Purified phage lysates were plated using the double agar overlay method. Each phage exhibited 1–3 mm turbid bullseye plaques, suggestive of a lysogenic life cycle. The presence of long, flexible tails supported their classification as Siphoviridae.

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