Pathway activity does not correlate with pathway dependency.

Metabolic pathway expression was correlated with gene dependency data and GSEA was run on the resulting correlation coefficients (see Fig 1). Then, results were filtered for results that had the same pathway expression and pathway dependency tested (e.g. Glycolysis dependency GSEA was queried against all correlations with Glycolysis expression). A) The resulting normalized enrichment scores (NES) are presented as a density plot for both Adherent RPMI and Adherent DMEM analyses. The distribution of NES is centered around 0. B) The NES for each self-dependency is plotted for Adherent RPMI and Adherent DMEM. Values for each self-dependency can be found in S2 Table.

(EPS)