## Parameter choices do not qualitatively change the biological interpretation of our information entropy measure.

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Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.

Cohen’s d statistic [29] comparing distributions of entropy values for cellular calcium activity of (a) stage 14 and stage 18 *Xenopus laevis* embryos, (b) stage 18 and stage 22 *Xenopus laevis* embryos, (c) stage 14 and stage 22 *Xenopus laevis* embryos, and (d) mature retrotrapezoid nucleus neurons from embryonic mice in pH 7.4 solution vs. pH 7.2 solution (data in (d) obtained from [15]). At large values of *n* and *k*, a sign change in d value occurs which is a technical artifact arising from there being more entries in the transition matrix than can be filled by data from our time series. The numerical values of d which generated this figure can be found in S2 Table.