Palmitate reduced FoxO1, ATGL and CGI-58 and increased PPARγ and its target gene G0S2 expression in HepG2 hepatocytes in a concentration-dependent manner.

(A) Palmitate reduced FoxO1, ATGL and CGI-58 but increased PPARγ and G0S2 mRNA expression. The mRNA levels were determined by a quantitative PCR analysis and were normalized to β-actin. (B) Palmitate reduced FoxO1, ATGL and CGI-58 and increased PPARγ and G0S2 protein expression. The protein expression levels were determined by a Western blot analysis. (C) The bands were quantified and normalized relative to β-actin. Each bar represents the means ± SDs (n = 3). *P < 0.05, **P < 0.01.