PAFAH1B2 regulates VSMC proliferation and contraction.

(A,B) A10 or 293T cells were transfected with empty vector or pCMV6-Pafah1b2, and cell proliferation was evaluated by the MTT assay. *P < 0.05 versus the vector-transfected group. (C) Transfection of VSMCs with PAFAH1B2 siRNA reduced the endogenous PAFAH1B2 mRNA levels. **P < 0.01 versus the control siRNA group. (D) VSMCs were transfected with control or PAFAH1B2 siRNA, and cell proliferation was evaluated by the MTT assay. *P < 0.05 versus the control siRNA group. (E) After transfection of VSMCs with PAFAH1B2 siRNA, cyclin D1 transcript levels were determined using RT-PCR. *P < 0.05 versus the control siRNA group. (F) VSMCs were transfected with control or PAFAH1B2 siRNA for 2 days, and then, collagen gel assay was performed. *P < 0.05 versus the control siRNA group. (G,H) After transfection of VSMCs with PAFAH1B2 siRNA, RhoA and Rock2 transcript levels were determined using RT-PCR. **P < 0.01 versus the control siRNA group. (I) Representative western blot images of RhoA and ROCK2 expression in PAFAH1B2 siRNA-transfected VSMCs. (J,K) Protein levels were quantified using ImageJ software. *P < 0.05 and **P < 0.01 versus the control siRNA group.