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Optimization of pseudovirus inoculum for neutralization assays.

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posted on 2021-03-10, 18:42 authored by Sabari Nath Neerukonda, Russell Vassell, Rachel Herrup, Shufeng Liu, Tony Wang, Kazuyo Takeda, Ye Yang, Tsai-Lien Lin, Wei Wang, Carol D. Weiss

Neutralizations were performed in 293T-ACE2.TMPRSS2s cells with mAb 10G6H5 (A and C) and a rabbit serum against the S1 subunit (B and D) using various pseudovirus inoculums. X-axis indicates mAb concentration (A and C) and serum dilution (B and D) used. Y axis indicates percentage inhibition compared to pseudovirus infection without mAb or serum treatment.

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