1471-2199-9-60-3.jpg (49.06 kB)
Off-target effects of siRNA specific for -2
figure
posted on 2011-12-30, 21:32 authored by Cordula Tschuch, Angela Schulz, Armin Pscherer, Wiebke Werft, Axel Benner, Agnes Hotz-Wagenblatt, Leticia Serra Barrionuevo, Peter Lichter, Daniel MertensDirected against were transfected in HeLa and (b) HEK cells. Expression of specific off-target genes (), non-target genes used as negative control () and genes unspecifically upregulated by antiviral cellular response () were measured by qPCR 48 h post-transfection. Expression values were normalized to the average of 2 housekeeping genes (, ). Correlation coefficients between the amount of siRNA transfected and the corresponding mRNA levels of the genes measured are given.
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Taken from "Off-target effects of siRNA specific for "
http://www.biomedcentral.com/1471-2199/9/60
BMC Molecular Biology 2008;9():60-60.
Published online 24 Jun 2008
PMCID:PMC2443166.
