Nucleotide changes in CVB3 infected Huh7 cells.

¹³C glucose isotope tracing study of mock and CVB3 infected Huh7 R19 cells (three replicates; one experiment; MOI 5). The cells were infected, lysed at 5,8 or 16 hpi and measured by LC-MS to identify metabolites. One sample was removed from analysis (one replicate of 5 hpi CVB3), because of a technical defect. A) The relative contributions of the different labelings are shown for eight representative nucleotides: ADP, ATP, UDP, UTP, CDP, CTP, GDP, GTP. B) The absolute level of N-carbanoyl-aspartate in Mock and CVB3 infected samples over time. The p-values of the absolute metabolite levels A) and B) were calculated using linear mixed effect models with an interaction of time and treatment and a random effect of replicate. For N-carbamoyl-aspartate, a normal distribution of the residuals could not be assumed and therefore a non-parametric linear mixed effect model with an interaction of time and treatment and a random effect of replicate was performed. Afterwards, a contrast analysis was done to calculate the p-values between specific groups. *p < 0.05, **p < 0.01, ***p < 0.001.

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