Nucleotide and amino acid alignments of the MpRTN4IP1L genes and MpRTN4IP1L proteins of Mprtn4ip1l mutants.

Mprtn4ip1l mutants were generated via CRISPR-Cas9 mutagenesis to target the MpRTN4IP1L gene. Wild type spores (Tak-1 x Tak-2) were transformed with Agrobacterium tumefaciens strains carrying the vectors containing the Cas9 gene and an sgRNA targeting MpRTN4IP1L: (A) sgRNA1, (B) sgRNA 2, (C) sgRNA3. Positive transformants were genotyped using Sanger sequencing to determine the mutations induced by the CRISPR-Cas9 complex. Predicted protein sequences were determined based on the mutations in each line. Nucleotide or protein sequences were aligned via the L-INS-i strategy using MAFFT version 7 [76]. The top row is the reference MpRTN4IP1L nucleotide sequence or the reference MpRTN4IP1L protein sequence (MpTak v6.1).

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