Nr-CWS TH treatment promote wound healing through MAPK/ERK, PI3K/AKT and JAK/STAT3 pathways.

A) The protein levels of VEGF and EGF at 14 days by western blotting, GAPDH served as protein loading control); B, C) The statistical analysis of VEGF and EGF. The data are expressed in mean ±SEM, n = 4 for each group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. D) Immunoblot for p-AKT, p-ERK, p-STAT3; the proteins amount of AKT, ERK, STAT3 served as loading control; E, F, G) Densitometric statistical analysis of p-AKT/AKT, p-ERK/ERK and p-STAT3/STAT3, respectively. GAPDH was used as internal parameter. Data presented as mean ± SEM n = 4 for each group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.