No HIV infection of PBMC origin CD14+ cells.

PBMC were infected with 2 × 10⁷ RNA copies/mL YFP-NL4–3(AD8). 2 days post-infection, cells were collected and stained with CD3 and CD14 antibodies, then analyzed for infection by detection of YFP positive cells in the CD3+ and CD14+ populations using flow cytometry. Shown are median and IQR for different blood donors. The median fraction of infected CD3+ gated CD4+ PBMC was 2.4% (IQR 1.7–3.2). No infected CD14+ monocytes were detected. The difference was significant (p-value is ****< 0.0001; Mann-Whitney U test). Brown circles denote blood donor 0020, red circles donor 0019, green donor 0049, and blue donor 0051.

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