ppat.1009712.s001.tif (565.12 kB)
Nigericin and ATP treatment induces the secretion of IL-1β in a dose-dependent manner in BMDMs.
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posted on 2021-07-29, 17:27 authored by Shivangi Rastogi, Sarah Ellinwood, Jacques Augenstreich, Katrin D. Mayer-Barber, Volker BrikenBMDMs were either left untreated (UT) or treated with 100ng/ml LPS for 4 h and then stimulated for 30 min with two different NLRP3 inflammasome activators, Nigericin and ATP at indicated doses. Cell supernatants were harvested after 30 min of stimulation and analyzed for (A, B) IL-1β release by ELISA. Data are representative of three independent experiments.
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pknF deletionIL -1βphosphokinase PknF Mycobacterium tu...NLRP 3 inflammasome activationhost cell NLRP 3 inflammasome activ...reactive oxygen speciesAIM 2 inflammasomebone marrow-derived macrophagesNLRP 3 inflammasomeMtbBMDMadaptor protein ASCNLRP 3 inflammasome inhibitionpyroptosiinflammasome activationIL -6 production
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