Niclosamide functions like BafA1 in inhibiting RBD uptake and neutralizing the endosomal pH.

A, B: For the endocytic assay experiment described in Fig 7A, images are shown in S8A Fig and quantification in Figs 7A and S8B, with total cell mean intensity shown in Fig 7A, number of endosomes shown in S8B(i) Fig and intensity per-endosome shown in S8B(ii) Fig. The number of RBD endosomes and dextran endosomes decrease, while transferrin endosomal intensity increases with increasing concentrations of Niclosamide. p-value table is indicated in S1 Table. C: For the pH estimation assay described in Fig 7C and7D, quantification of endosomal FITC intensities, TMR intensities and FITC/TMR endosomal ratio is shown in S8C. A dose-dependent increase in FITC endosomal intensity, as well as ratio, is seen with increasing Niclosamide concentrations. p-value table is indicated in S1 Table. Data representation in B, C is as described in Fig 2. Scale bar shown in A is 40μm.

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