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Ni-IMAC purification of GPR3 and S1P1 analyzed by SDS-PAGE after Coomassie-staining (blue) and verified by western blot (grey/black).

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posted on 2021-02-24, 18:38 authored by Bastian Heim, René Handrick, Marcus D. Hartmann, Hans Kiefer

Both proteins were detected with an anti-(His)6 primary antibody (mouse; Qiagen AG, Hilden, Germany) and an anti-mouse HRP labeled secondary antibody. GPR3 F1 –F3: Ni-IMAC elution fraction 1 to 3; S1P1 F1 –F2: Ni-IMAC elution fraction 1 and 2. Bands (monomer) resulting from GPR3 and S1P1 are indicated by arrows.

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    membrane proteincoli inclusion bodies Aimingcoli inclusion bodiesCPMphase crystallization screeningreceptor GPR 3. Refolded receptorsrefoldingGPCR productionG protein-coupled receptorsthermostability analysisSuccessful expressionsphingosine -1-phosphate receptor S...

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