ppat.1009352.s009.tif (1.09 MB)
Neutralisation of SARS-CoV-2 by monoclonal antibodies in the PCR-based neutralisation assay.
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posted on 2021-02-26, 18:43 authored by Kuan-Ying A. Huang, Tiong Kit Tan, Ting-Hua Chen, Chung-Guei Huang, Ruth Harvey, Saira Hussain, Cheng-Pin Chen, Adam Harding, Javier Gilbert-Jaramillo, Xu Liu, Michael Knight, Lisa Schimanski, Shin-Ru Shih, Yi-Chun Lin, Chien-Yu Cheng, Shu-Hsing Cheng, Yhu-Chering Huang, Tzou-Yien Lin, Jia-Tsrong Jan, Che Ma, William James, Rodney S. Daniels, John W. McCauley, Pramila Rijal, Alain R. Townsend(A) An illustrative example of measuring Ct value of virus signal in the tissue-culture supernatant of SARS-CoV-2 infected Vero E6 cells using an E gene-based real-time reverse-transcription PCR assay. The right shift of the amplification plot reflects the increase in Ct value and the decrease of viral load. (B) Neutralisation data for MAbs EW 9C, EY 6A, FD 5D, FD 11A and FI 3A. Increases in Ct value indicate decreases in virus loads. Each unit increase indicates a 2x reduction resulting from the presence of MAb. A 10x increase in Ct = 1,024-fold reduction of virus load.
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3 weeksantibody responseSARS-CoV -2 infectionACE 2-blocking responsePotent humoral responsesCOVID -19 patientscross-inhibiting clustersneutralising antibodiesplasmablast-derived IgGAnti-RBD MAbsplasmablast responses32 anti-spike MAbsvirus spike glycoproteinACE 2non-competing pairsplasmablast responsememory B cellsanti-non-RBD S 1plasmablast-derived MAbsanti-receptor-binding domainACE 2-blocking anti-RBD antibodies
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