pntd.0011878.s008.tif (1.34 MB)
Neighbour-joining phylogenetic tree based on the DNA sequence of the nuclear small-subunit (18S) rRNA, the Rns gene of Acanthamoeba isolates.
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posted on 2024-01-02, 19:15 authored by Binod Rayamajhee, Mark Willcox, Fiona L. Henriquez, Ajay Kumar Vijay, Constantinos Petsoglou, Gauri Shankar Shrestha, Hari Kumar Peguda, Nicole CarntTwo Acanthamoeba isolates (AK1—blue coloured and AK11-yellow coloured) of this study belonged to genotype T4F subclade. The reference genotype sequences were obtained from the NCBI database.
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primary human macrophagesincreasingly important pathogenwild type strainstrain pao1 (<increased neutrophil responsealkyne – functionalizednaturally acquired intracellulardomestic tap waterpseudomonas aeruginosa live intracellular bacteriarats eyes resulteddomestic watermotile strainaeruginosa – alanineclinical responsehousehold waterintracellular survivalintracellular bacteriavivo situ p acanthamoeba various microorganismsspecies recoveredseen throughoutpotentially attributedmetabolically activemay indicategreater degreegenotype t4ffish ),findings indicateenvironmental hostelectron microscopycorneal swabscorneal infectionschallenged intrastromallyanimal modelalso becomingalkdala ).ak patients>< 005 ).
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