NPC1 KO validation in Vero cells.

(A) NPC1 detection in Vero or Vero NPC1 KO cell lines (B) Indirect immunofluorescence shows NPC1 in green detected with a specific antibody against NPC1. Scale bar: 25 μm (C-D) NPC1 KO cells depicts dilated endosomes detected in red with the acidic probe Lysotracker. Cholesterol stained with Filipin III (in blue) accumulates in dilated vesicles, similarly as it occurs in cells pre-treated with U18666A drug columns on the right-hand side. Scale bar: 10 μm. (E) Infection of Vero and NPC1-KO-Vero cells with recombinant VSV (rVSV) pseudotyped with Ebolavirus Glycoprotein (EBOV-GP) Mayinga strain or VSV-G 24 h. Cells were lysed 24 h post-infection and assayed for luciferase expression. Percentages of infected cells were determined by setting the number of RLU in Vero cells to 100% for each envelope.

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