Mutants lacking CgFld-LPs are not sensitive to thermal stress.
A. Serial dilution spotting analysis showing growth of C. glabrata mutants deleted for single or multiple CgFld-LP genes on indicated stress conditions. Duroquinone (DQ) and cumene hydroperoxide (CHP) were used at a concentration of 450 μM and 40 μM, respectively. Plates were incubated at 30οC unless indicated otherwise, and images were captured after 24 h for thermal stress (42οC), and 48 h for DQ and CHP. The Q-KO strain lacks four flavodoxin-like proteins, CgPst2, CgRfs1, CgPst3 and CgYcp4. B. Serial dilution spotting analysis showing CgPST2-mediated complementation of menadione (MD; 95 μM) and benzoquinone (BQ; 4 mM) sensitivity of Cgpst2Δ and Q-KO strains. ‘V’ refers to empty vector. Plates were incubated at 30οC, and images were captured after 24 h and 48 h for MD and BQ stresses, respectively.
(TIF)