ppat.1009430.g001.tif (1.43 MB)
Monocytes of Malian adults exhibit reduced P. falciparum-inducible inflammatory cytokine production and increased IL-10 production.
figure
posted on 2021-04-06, 17:24 authored by Rajan Guha, Anna Mathioudaki, Safiatou Doumbo, Didier Doumtabe, Jeff Skinner, Gunjan Arora, Shafiuddin Siddiqui, Shanping Li, Kassoum Kayentao, Aissata Ongoiba, Judith Zaugg, Boubacar Traore, Peter D. CromptonPBMCs were collected cross-sectionally from an age-stratified cohort in Mali before the malaria season when all subjects were negative for Pf infection by PCR; and also, from malaria-naïve U.S. adults. Monocytes were isolated from thawed PBMCs and stimulated with Pf-iRBC lysate at a ratio of 1 monocyte to 30 iRBCs. After 24 hours, cell culture supernatants were analyzed to quantify secreted (A) TNF, (B) IL-1β, (C) IL-6 and (D) IL-10. Median values are indicated by a horizontal line. P-values are from pairwise Mann-Whitney tests with a Bonferroni adjustment for all 10 pairwise comparisons. Nonsignificant p-values are not shown.
