Monocytes from HAM/TSP patients present greater IL-12 and MIP-1α response to stimulation.

(A) Gating strategy for identification of the three monocyte subpopulations using CD16 and CD3-14-15-19 antibodies. (B) Frequency of innate subsets in the clinical groups evaluated in monocytes subsets (classical monocytes, intermediate monocytes and non-classical monocytes). (C) tSNE analysis of monocyte subset frequencies in whole blood samples at steady state and after TLR7-stimulation with R848. (D) Frequency of the cells producing IFNα, IL-12, MIP-1α and TNFα at steady state and after R848 treatment in the different monocyte subsets. HD: healthy donor; AC: asymptomatic carriers; and HAM/TSP: HTLV-1 associated myelopathy/Tropical spastic paraparesis. Statistical significance was determined using one-way ANOVA followed by Turkey post-hoc test. * p-value ≤ 0.05; ** p-value ≤ 0.01; *** p-value ≤ 0.001. (D) Pie-chart of the Boolean analysis for the cytokine production in monocyte subsets at steady state (SS) and after TLR7 stimulation (R848).