pone.0242443.s001.tif (4.25 MB)

Mitochondrial content is similar between control and IIM patients.

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posted on 2020-11-20, 18:26 authored by Carla Basualto-Alarcón, Félix A. Urra, María Francisca Bozán, Fabián Jaña, Alejandra Trangulao, Jorge A. Bevilacqua, J. César Cárdenas

(A) Human skeletal muscle biopsies obtained from controls and IIM patients were labeled with a specific antibody against the outer membrane mitochondrial protein VDAC. Equally sized "Regions Of Interest” (ROIs) were analyzed with Image J, and the area was expressed in pixel units. No differences were observed between controls and IIM patients. Controls n = 3; Patients n = 4. (B). Mitochondrial complexes (I, III, IV and V) were analyzed by Western Blot in tissue samples from human skeletal muscle biopsies obtained from control and IIM patients. No differences were observed. Controls n = 3; IIM n = 3. Mean ± SEM.