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Mitigation strategy.

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posted on 11.10.2021, 17:42 authored by Matthew Z. DeMaere, Aaron E. Darling

A simple QA directed mitigation strategy, where Hi-C sequencing libraries are assessed for quality (SNR) using a small-scale pilot sequencing run. Very poor quality libraries can be rejected, while accepted libraries go on to deeper sequencing. The total required yield of paired reads can be calibrated by the inferred SNR. Given sufficient starting material, Hi-C library generation can be reattempted when previous libraries are abandoned.