ppat.1008937.s003.tiff (2.02 MB)
Minigenome assays were performed in 293T cells.
figure
posted on 2021-06-01, 17:41 authored by Daniel H. Goldhill, Ada Yan, Rebecca Frise, Jie Zhou, Jennifer Shelley, Ana Gallego Cortés, Shahjahan Miah, Omolola Akinbami, Monica Galiano, Maria Zambon, Angie Lackenby, Wendy S. BarclayPol I–firefly luciferase minigenome reporter, at 0.08 μg and PCAGGS-Renilla, at 0.1 μgwere transfected with PCAGGS plasmids coding for wildtype and mutated polymerase subunits (PB1, PB2 and PA) and NP at 0.08, 0.08, 0.04 and 0.12 μg respectively at 37°C or 33°C. Luciferase signal was read 24 hours post-transfection. Polymerase activity is given as a ratio Firefly to Renilla signals.
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