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Michaelis-Menten plots of enzymatic activity for GST-CBU1241 in relation to substrates OAA (A) and NADH (B).

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posted on 2021-08-13, 17:35 authored by Janine Hofmann, Mebratu A. Bitew, Miku Kuba, David P. De Souza, Hayley J. Newton, Fiona M. Sansom

Enzyme specific activity (μmol/mg per mg protein) measured for varying concentrations of OAA concentrations at 0.5 mM NADH (A), and varying NADH concentrations at 0.6 mM OAA (B). Error bars represent standard deviation from the mean from at least three independent experiments. Linear regression line of best fit for Michaelis-Menten kinetic equation is plotted in grey for NADH (B).

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