ppat.1012036.s008.tif (1.09 MB)
Metabolomic analysis of 6-MP and CPU treated cells.
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posted on 2024-03-08, 18:21 authored by Lonneke V. Nouwen, Martijn Breeuwsma, Esther A. Zaal, Chris H. A. van de Lest, Inge Buitendijk, Marleen Zwaagstra, Pascal Balić, Dmitri V. Filippov, Celia R. Berkers, Frank J. M. van Kuppeveld13C-glucose isotope tracing study in control- and 6-MP or CPU treated HeLa R19 cells (three replicates; one experiment). Cells were exposed to different concentrations of 6-MP or CPU (30 μM, 100 μM, 300 μM, 1000 μM). The cells were lysed after at 6h and measured by LC-MS to identify metabolites and quantify the different isotopologues. The different isotopologues are not distinguished in this Figure. Heatmap showing log2 fold changes of intracellular metabolites compared to control-treated cells.
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