Metabolic follow-up of animal models and oscillatory potential analysis.

A, Follow-up of body weight and blood glucose levels, glucose tolerance test, and insulin tolerance test in control-diet (n = 75) and high-fat diet-fed (n = 75) mice for 12 weeks. B, Body weight, blood glucose levels, glucose tolerance test, and insulin tolerance test in lean (n = 20) and spontaneously obese (n = 20) Neotomodon alstoni mice. In both models, mice have higher glycemia than control mice at every time point (P < 0.05), suggesting reduced insulin sensitivity. This was confirmed by insulin tolerance tests, which showed a lower fall in blood glucose in response to insulin in obese mice as compared with control mice. C, Blood glucose level follow-up in rats after 4, 6, 8, or 12 weeks of streptozotocin (n = 40) and vehicle (n = 40) treatment. Values, mean ± s.d. * indicates P values < 0.05 determined by a two-sample Student’s t-test in B (body weight and glycemia) and by a mixed ANOVA followed by Bonferroni test everywhere else. D, Illustrative ERG (top) and oscillatory potentials (OP, bottom) in control-diet and high-fat diet-fed mice for 12 weeks, measured in response to a light flash of 7.72 (cd.s)/m² (arrow) under dark-adapted conditions. E, Temporal monitoring (0 to 12 weeks, as indicated) of the average amplitude and implicit time of OP1, OP2, OP3, and OP4 in control (n = 10) and high-fat diet fed mice (n = 12) under dark-adapted conditions at increasing light intensities (0.02, 0.24, 2.45, and 7.72 (cd.s)/m². CD, control diet. HFD, high-fat diet. n.s., not significant (P > 0.05).

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